Fig. 5

Circ_0002483 acted as a sponge of miR-125a-3p in LUAC cells. A Schematic representation of the binding sites of miR-125a-3p with WT or Mut circ_0002483 3′UTR. B Estimation of luciferase activities of WT or Mut circ_0002483 3′UTR after co-treatment with miR-125a-3p mimics or inhibitor and WT or Mut circ_0002483 3’UTR reporter vectors in A549 and PC9 cells. C qRT-PCR analysis of the expression levels of miR-125a-3p after transfection with its mimics and inhibitor in A549 and PC9 cells. D qRT-PCR analysis of the expression levels of miR-125a-3p after transfection with circ_0002483 overexpression vectors or si-circ_0002483 in A549 and PC9 cells. E, F RIP analysis of the enrichment levels of circ_0002483 and miR-125a-3p pulled down from Ago2 protein in A549 and PC9 cells. G CCK-8 analysis of cell proliferation viability after co-transfection with circ_0002483 overexpression vectors and miR-378a-3p mimics in A549 cells or si-circ_0002483 and miR-125a-3p inhibitor in PC9 cells. Data shown are the mean ± SEM of three experiments. *P < 0.05, **P < 0.01, ***P < 0.001