Fig. 6

miR-125a-3p reversed circ_0002483-caused upregulation of CCL4-CCR5 axis. A Schematic representation of the binding sites of miR-125a-3p with WT or Mut CCL4 3′UTR. B Estimation of luciferase activities of WT or Mut CCL4 3′UTR after co-treatment with miR-125a-3p mimics and WT or Mut CCL4 3’UTR reporter vectors in A549 and PC9 cells. C, D TCGA analysis of the expression levels of CCL4 and CCR5 in LUAC tissue samples (n = 515). E Pearson correlation analysis of the correlation of miR-125a-3p with CCL4 and CCR5 expression in LUAC tissue samples. F, G qRT-PCR and Western blot analysis of the expression levels of CCL4 and CCR5 after co-transfection with circ_0002483 overexpression vectors and miR-125a-3p mimics in A549 cells. Data shown are the mean ± SEM of three experiments. *P < 0.05, **P < 0.01, ***P < 0.001