Fig. 9

FAM49B-knockdown BC cells are more sensitive to doxorubicin. A FAM49B mRNA expression was lower in anthracycline-sensitive BC samples, as per ROC Plotter analysis. B High FAM49B mRNA level was associated with decreased RFS in BC patients who had received chemotherapy. C The dot matrix of Annexin V/PI double staining was generated after scr- and FAM49B-shRNA MDA-MB-231 cells were treated or not treated with 200 ng/mL of doxorubicin for 24 h (n = 3). scr- and FAM49B-shRNA MDA-MB-231 cells were injected into mice, as described previously. Tumor growth (D) and tumor volume (E) were measured on the indicated days. The arrows showed the time of doxorubicin injection. F FAM49B-shRNA or scr-shRNA were transfected into MDA-MB-231 cells treated with various doses of doxorubicin for 24 h. Levels of ELAVL1, Rab10, TLR4, p-p65, XIAP, survivin, c-caspase 3, and c-PARP1 in MDA-MB-231 cells were quantified via western blotting (n = 3). Results are presented as the mean ± SD. The statistical significance was assessed by Student’s t-test; *p < 0.05, **p < 0.01