Fig. 5

Overexpression of SNHG12 reduced the inhibition of overexpression of miR-140-3p on proliferation, invasion, and migration of GC cells. The lentiviral overexpression vector of SNHG12 was infected with AGS cells. A The expression of SNHG12 was detected using RT-qPCR. Joint intervention with lentiviral overexpression vector of miR-140-3p was performed. The proliferation of cells was detected using CCK-8 assay (B) and colony formation assay (C). D, E The invasion and migration of cells were detected by Transwell assays. The experiment was repeated three times independently. Data were presented as mean ± standard deviation. Comparison of data among panels in A, C, D, E was performed using the t-test. Comparison of data in B was performed using two-way ANOVA, followed by Tukey's multiple comparisons test. **p < 0.01. LV-oe-miR: The lentiviral overexpression vector of miR-140-3p; LV-SNHG12: The lentiviral overexpression vector of SNHG12; LV-NC: The lentiviral overexpression vector of NC