Fig. 7

SNHG12 up-regulated the transcription of FAM83B by binding to the RNA-binding protein HuR. A The binding probability between HuR and FAM83B was predicted using RNA–Protein Interaction Prediction (RPISeq) database. B The binding between HuR and FAM83B was analyzed by RIP assay. C–E The expression of FAM83B in gastric adenocarcinoma was predicted by the Starbase, UALCAN, and GEPIA database. F, G The mRNA level of FAM83B in GC tissues and cells was detected by RT-qPCR. H Relevance between SNHG12 and FAM83B by Pearson. I, J mRNA level and half-life period of FAM83B in AGS cells were detected by RT-qPCR. The experiment was repeated 3 times independently. Data in panels A, B, G, I were presented as mean ± standard deviation. Comparison between data in panel F was analyzed using the t-test. Comparisons among data in panels G/I were analyzed using one-way ANOVA and comparison among data panel J was analyzed using two-way ANOVA followed by Tukey's multiple comparisons test. **p < 0.01. sh-NC: NC shRNA: sh-SNHG12: SNHG12 shRNA. pc-NC: NC pcDNA; pc-HuR: HuR pcDNA