Fig. 3From: Upregulation of SNTB1 correlates with poor prognosis and promotes cell growth by negative regulating PKN2 in colorectal cancerSNTB1 knockdown inhibits colorectal cancer (CRC) cell growth. HCT116 and RKO cells were transduced with lentivirus encoding either anti-SNTB1 small hairpin RNA (sh-SNTB1) or control shRNA (sh-Ctrl). a The mRNA levels of SNTB1 were determined by qPCR, GAPDH was used as an internal control. *P < 0.05. b, c The protein levels of SNTB1 were determined by Western-blot analysis. The representative images of SNTB1 and GAPDH are shown (b) and were quantitated using ImageLab software (c). GAPDH was used as an internal control and normalized to GAPDH. *P < 0.05. d The cell viability of CRC cells was determined by the CCK-8 assay. Data were normalized to the viability on Day 1 and are represented as fold change. *P < 0.05. e The colony formation assay was performed to determine cell survival. The images were obtained and the number of colonies formed were determined and normalized to the survival of control cells. *P < 0.05Back to article page