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Fig. 3 | Cancer Cell International

Fig. 3

From: Upregulation of SNTB1 correlates with poor prognosis and promotes cell growth by negative regulating PKN2 in colorectal cancer

Fig. 3

SNTB1 knockdown inhibits colorectal cancer (CRC) cell growth. HCT116 and RKO cells were transduced with lentivirus encoding either anti-SNTB1 small hairpin RNA (sh-SNTB1) or control shRNA (sh-Ctrl). a The mRNA levels of SNTB1 were determined by qPCR, GAPDH was used as an internal control. *P < 0.05. b, c The protein levels of SNTB1 were determined by Western-blot analysis. The representative images of SNTB1 and GAPDH are shown (b) and were quantitated using ImageLab software (c). GAPDH was used as an internal control and normalized to GAPDH. *P < 0.05. d The cell viability of CRC cells was determined by the CCK-8 assay. Data were normalized to the viability on Day 1 and are represented as fold change. *P < 0.05. e The colony formation assay was performed to determine cell survival. The images were obtained and the number of colonies formed were determined and normalized to the survival of control cells. *P < 0.05

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