Fig. 7

SNTB1 knockdown increases PKN2 protein expression and decreases p-ERK and p-AKT expression. HCT116 cells were transduced with lentivirus encoding either anti-SNTB1 shRNA (sh-SNTB1) or control shRNA (sh-Ctrl), and iTRAQ was used to identify DEPs. A hierarchical clustering plot (a) and a volcano plot (b) were used to identify DEPs (fold change ≥ 1.5, P < 0.05). c KEGG pathway enrichment analysis was performed to identify functionally related gene pathways. The top 20 enriched signaling pathways are shown. d Protein-protein interaction (PPI) network among DEPs was constructed. Red represent up-regulated proteins, while green represent down-regulated proteins. e The protein levels of PKN2 in HCT116 cells after SNTB1 knockdown were determined by Western-blot analysis. f The protein levels of p-ERK and ERK in HCT116 cells after SNTB1 knockdown were determined by Western-blot analysis. g The protein levels of p-AKT and AKT in HCT116 cells after SNTB1 knockdown were determined by Western-blot analysis. The representative images are shown in the upper panel and its expression was quantitated using ImageLab software in the lower panel. GAPDH was used as an internal control. The protein expression in sh-Ctrl was set as 1. The protein expression is presented as the fold change relative to the sh-Ctrl group *P < 0.05. All experiments were performed in triplicate