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Fig. 8 | Cancer Cell International

Fig. 8

From: Upregulation of SNTB1 correlates with poor prognosis and promotes cell growth by negative regulating PKN2 in colorectal cancer

Fig. 8

SNTB1 knockdown suppresses cell proliferation and induces cell apoptosis dependent on PKN2. HCT116 cells were transfected with si-SNTB1 or si-PKN2, or combination of si-SNTB1 and si-PKN2, or their related control siRNA (si-Ctrl). a The protein levels of SNTB1 (left) and PKN2 (right) in HCT116 cells were determined by Western-blot analysis. The representative images of PKN2, SNTB1 and GAPDH were shown and were quantitated using ImageLab software. GAPDH was used as an internal control and normalized to GAPDH. *P < 0.05, #P < 0.05 vs. si-PKN2. b The cell viability of CRC cells was determined by the CCK-8 assay. The cell viability in si-Ctrl was set as 1. Data were normalized to the viability of si-Ctrl and represented as the fold change. *P < 0.05 vs. si-Ctrl, #P < 0.05 vs. si-PKN2. c The colony formation assay was performed to determine cell survival. The images were taken and the number of colonies were calculated and normalized to the survival of control cells. *P < 0.05, #P < 0.05 vs. si-PKN2. d The cell cycle progression in HCT116 cells was determined by PI staining and FACS analysis. Representative plots (left panels) and percentage of cells (right panel) at different stages (G0/G1, G2/M and S phases) are presented. *P < 0.05. e Apoptosis of HCT116 cells was determined by Annexin V staining and FACS analysis. Representative plots (left panels) and percentage of apoptotic cells (right panel) are presented. *P < 0.05

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