Fig. 5
From: Identification of key genes for HNSCC from public databases using bioinformatics analysis
Expression levels of the three key genes in different HNSCC tissues. A Real-time PCR of CEACAM5, CEACAM6 and CLCA4 in laryngeal carcinoma, hypopharyngeal carcinoma and oropharyngeal carcinoma. The relative mRNA expression levels of these three genes in most HNSCC tissues were lower than those in adjacent noncancerous tissues (scale bars represent the mean ± SEM, Student’s t-test, *P < 0.05, **P < 0.01). B IHC of CEACAM5, CEACAM6 and CLCA4 in laryngeal carcinoma, hypopharyngeal carcinoma and oropharyngeal carcinoma. The protein expression levels of these three genes were lower than those in adjacent noncancerous tissues. C Semiquantification analysis of immunohistochemistry of each laryngeal carcinoma, hypopharyngeal carcinoma and oropharyngeal carcinoma tissue and paired adjacent normal tissue. Most of the tumour tissues in this study showed less staining than adjacent normal tissues. Abbreviations: LC: laryngeal carcinoma; HPC: hypopharyngeal carcinoma; OPC: oropharyngeal carcinoma