Fig. 2

miR-769-5p promotes the proliferation of OS cells in vitro and in vivo. A The expression of miR-769-5p in MG63 and 143B cells transfected with miR-769-5p mimics or miR-769-5p inhibitor; B-E CCK-8 (B), EdU (C, D) and colony-formation assays(C, E) were used to detect the effect of miR-769-5p on proliferation in vitro; F Western blot analysis of cell-cycle-related proteins following miR-769-5p mimics and inhibitor; G Images of tumors obtained from mice treated with miR-769-5p mimics and miR-769-5p inhibitor and their negative controls; n = 5 mice/group; H, I Tumor weight (H) and volume (I) were calculated; n = 5 mice/group; J Representative H&E-stained tumors and immunohistochemical staining of Ki-67 from mice in different groups; n = 5 mice/group; K Representative images of tumors were obtained by the IVIS imaging system; n = 5 mice/group; L Quantification of the luciferase is shown. Data are presented as the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001