Fig. 7

Role of OSM in migration, invasion and proneural-mesenchymal transition of glioblastoma cells. a The wound healing of glioblastoma cells using scratch assay. Scale bar, 50 μm. Magnification, 200×. b Statistical analysis of wound healing rates. c Invasive potential of glioblastoma cells in matrigel invasion assay. Scale bar, 50 μm. Magnification, 200×. d Statistical analysis of the number of cell counts in each field of view. e mRNA levels of mesenchymal signature genes in control and OSM-treated group. f mRNA levels of proneural signature genes in control and OSM-treated group. g Representative immunoblots showing levels of CD44, FN1, CHI3L1, CD24, DLL3 and OLIG2 in U251 cells. β-ACTIN was the loading control. h ChIP-seq analysis of the binding site of STAT3 to OSMR, LIFR and IL6R. i mRNA levels of OSM-related receptors (OSMR, LIFR and IL6R) in control and OSM-treated group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001