Fig. 8

Impact of STAT3 signaling pathway in OSM-induced glioblastoma cell migration, invasion and proneural-mesenchymal transition. a The wound healing of OSM-treated glioblastoma cells before and after inhibition of STAT3. Scale bar, 50 μm. Magnification, 200×. b Statistical analysis of wound healing rates. c Invasive potential of glioblastoma cells in matrigel invasion assay. Scale bar, 50 μm. Magnification, 200×. d Statistical analysis of the number of cell counts in each field of view. e mRNA levels of mesenchymal signature genes in control, OSM-treated, and STAT3-inhibited groups. f mRNA levels of proneural signature genes in three groups. g Representative immunoblots showing levels of CD44, FN1, CHI3L1, CD24, DLL3 and OLIG2 in U251 cells. β-ACTIN was the loading control. h Graphical illustration of the OSM positive feed-forward loop in tumor cell. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001