Fig. 1

KSHV successfully infects SH-SY5Y cells. A Fluorescence microscope was used to confirm the successful infection. SH-SY5Y cells were infected with KSHV for 72 h and re-named as SK-RG cells. GFP fluorescence was used to confirm infection (magnification, ×40 and ×200). Some lytic state cells expressed RFP spontaneously. B The cell morphology was observed by microscope at ×100, ×200 and ×400. C The KSHV DNA copy number was detected by TaqMan real-time PCR. Supernatants were collected from lytic-induced islk.219 and SK-RG cells, after confirming infection, and cells were cultured for 7 days