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Fig. 7 | Cancer Cell International

Fig. 7

From: Kaposi’s sarcoma-associated herpesvirus infection promotes proliferation of SH-SY5Y cells by the Notch signaling pathway

Fig. 7

KSHV infection increased the expression of Notch 1 signaling pathway proteins and interfering Notch1 inhibits the proliferation of the cells. A Real-time PCR was used to analyze the mRNA expression level of Notch1 in SK-RG and SH-SY5Y cells. B, C The expression level of Notch1, NICD, RBP-Jĸ, and Hes1 were detected by western blots in SK-RG and SH-SY5Y cells. D RNA interference was used to interfere with the expression of Notch 1 in SK-RG cells, and then the efficiencies of Notch 1 interference was investigated using the real time-PCR. E, F Detection of Notch 1, NICD, RBP‐Jĸ, and Hes1 protein expression levels by western blot in si-Notch1 and NC group. G MTT assay was used to detect the proliferation ability in si-Notch1 and NC group. H The cell proliferation was detected by Ki-67 staining in si-Notch1 and NC group (P < 0.01)

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