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Fig. 1 | Cancer Cell International

Fig. 1

From: LncRNA ASAP1-IT1 enhances cancer cell stemness via regulating miR-509-3p/YAP1 axis in NSCLC

Fig. 1

ASAP1-IT1was elevated in NSCLC tissues and knockdown of ASAP1-IT1inhibited the cancer cell stemness of NSCLC. A H&E staining of NSCLC tissues and adjacent tissues. B qRT-PCR analysis of ASAP1-IT1 expression in NSCLC tissues and adjacent tissues, *p < 0.01 compared with adjacent tissues (n = 83); C qRT-PCR analysis of ASAP1-IT1 expression in NSCLC cells and normal cells, *p < 0.01 compared with BEAS-2B cells; D qRT-PCR analysis of ASAP1-IT1 expression in spheres of A549, *p < 0.01 compared with parental group. E qRT-PCR on the expression of stemness-associated gens (SOX2, CD44, and CD133) in stemness‐enriched cell spheres and parental cells, *p < 0.01 compared with parental group. F qRT-PCR analysis of ASAP1-IT1 levels after 48 h infection with pcDNA-sh-NC or pcDNA-sh-ASAP1-IT1 in A549-derived stem cells, *p < 0.01 compared with pcDNA-sh-NC. G qRT-PCR analysis on stemness-associated gens (SOX2, CD44, and CD133) after 48 h infection with pcDNA-sh-NC or pcDNA-sh-ASAP1-IT1 in A549-derived stem cells, *p < 0.01 compared with pcDNA-sh-NC. H, I Number of tumor spheres in ASAP1-IT1 knockdown A549-derived stem cells, *p < 0.01 compared with pcDNA-sh-NC. J Flow cytometry analysis of cell cycle in A549 stem cells post 48 h infection, *p < 0.01 compared with pcDNA-sh-NC

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