Fig. 2

Knockdown of ASAP1-IT1suppressed cisplatin resistance and cell growth of cancer stem cells and interfering ASAP1-IT1promoted apoptosis. qRT-PCR analysis of ASAP1-IT1 expression in A A549 cells post incubation with 4 μM cisplatin at different times (0 h, 6 h, 12 h, 18 h, 24 h, 30 h), *p < 0.01 compared with 0 h group and B A549 and cisplatin-resistant A549/R cells, *p < 0.01 compared with A549. C CCK-8 assay on the cell viability of ASAP1-IT1 knockdown A549/R cells incubated with cisplatin at various concentration (0 μM, 4 μM, 8 μM, 16 μM, 32 μM), *p < 0.01 compared with pcDNA-sh-NC. D, E Annexin V-FITC/PI staining of A549-dereived stem cells 48 h post infection, *p < 0.01 compared with pcDNA-sh-NC. F qRT-PCR analysis of expression of apoptosis-associated genes (Bax and Bcl-2) 48 h after infection in A549-dereived stem cells, *p < 0.01 compared with pcDNA-sh-NC. G, H Colony formation assay on cell growth of A549-dereived stem cells after 15 days of culture, *p < 0.01 compared with pcDNA-sh-NC. I qRT-PCR analysis on the expression of proliferation-associated genes (Cyclin A1, Cyclin B1, and PCNA) after 48 h infection in A549-dereived stem cells, *p < 0.01 compared with pcDNA-sh-NC