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Fig. 3 | Cancer Cell International

Fig. 3

From: LncRNA ASAP1-IT1 enhances cancer cell stemness via regulating miR-509-3p/YAP1 axis in NSCLC

Fig. 3

ASAP1-IT1 interacted with miR-509-3p. A The potential binding sites of miR-509-3p on ASAP1-IT1 predicted by LncBook. B qRT-PCR analysis of miR-509-3p expression in NSCLC tissues and adjacent tissues (n = 83), *p < 0.01 compared with adjacent tissues. C The correlation of ASAP1-IT1 and miR-509-3p in NSCLC tumors (n = 83). D qRT-PCR analysis of miR-509-3p in A549 cells 48 h post infection with pcDNA, pcDNA-ASAP1-IT1 (ASAP1-IT1), and pcDNA-sh-ASAP1-IT1 (sh-ASAP1-IT1), &p < 0.01 compared with pcDNA. E qRT-PCR analysis of miR-509-3p expression in A549 cells 48 h after transfection with mimic miRNAs, *p < 0.01 compared with Mock. F Luciferase reporter assay onmiR-509-3p on luciferase activity of pmirGLO-ASAP1-IT1 WT (wild type) and pmirGLO-ASAP1-IT1 Mut (mutant) 48 h post transfection with mimic miRNAs, *p < 0.01 compared with Mock. G qRT-PCR analysis of ASAP1-IT1 expression in A549 cells 48 h after infection, *p < 0.01 compared with Mock. H qRT-PCR analysis of RNA content after RIP assay in A549 cells, *p < 0.01 compared with anti-IgG

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