Fig. 4

CSC induced BiP expression in OSCC cells by downregulating miR-30a. A YD38 and SCC25 cells were treated with either vehicle control or 120 μg/ml CSC for 48 h. The expression of miR-30a was evaluated using qRT-PCR. B Sequence alignment of miR-30a with the target sequence on the 3-UTR of BiP transcript. C The direct target relationship between miR-30a and BiP was confirmed using a luciferase assay. OSCC cells transfected with negative control (miR-NC) or miR-30a mimics were further transfected with pBiP-3'UTR plasmids followed by CSC treatment. Luciferase activity was detected using a luciferase assay. All data are presented as the mean ± SEM. SEM, error bar. *P < 0.05 by Student’s t test or one-way ANOVA followed by Bonferroni’s post hoc test