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Fig. 5 | Cancer Cell International

Fig. 5

From: Dysregulation of the miR-30a/BiP axis by cigarette smoking accelerates oral cancer progression

Fig. 5

miR-30a was involved in CSC-induced BiP expression and OSCC malignancy. YD38 and SCC25 cells transfected with miR negative control (miR-NC) or miR-30a mimics were treated with 120 μg/ml CSC for 48 h. A and B The expressions of BiP and VEGF were examined by qRT-PCR and Western blot analysis. C The secretion of VEGF from OSCC cells was detected by ELISA. D The invasion ability was evaluated using a Transwell invasion assay. OSCC cells after the indicated treatments were allowed to invade through Matrigel-coated inserts for 24 h. The invaded cells were then fixed with methanol and stained with propidium iodide (PI). Representative images are shown (Scale bar, 50 μm). The amount of PI-stained cells was determined using ImageJ software. E The effect of OSCC cell-derived conditioned media (CM) on the angiogenic activities of HUVECs was determined using a tube formation assay. Representative images show the tube formation of HUVECs in CM from OSCC cells at 6 h (Scale bar, 100 μm). Total tube length was analyzed using an angiogenesis analyzer for ImageJ software. GAPDH was used as the internal control. All data are presented as the mean ± SEM. SEM, error bar. *P < 0.05 by one-way ANOVA followed by Bonferroni’s post hoc test

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