Fig. 1

LC–MS/MS-based quantitative phosphoproteomic profiling of phosphorylation in SaOS-2 osteosarcoma cells. a Protein expression profiling of osteosarcoma cells in the Con group, Lob group, and IL-6 group. Twenty micrograms of protein was loaded, and the gel was stained with Coomassie Blue R-250 after electrophoresis. b Base peak chromatograms demonstrating the correspondence of peptides in each group. c Phosphorylation patterns of the amino acids serine, threonine, and tyrosine. d Experimental scheme of the quantitative phosphoproteomic analysis of osteosarcoma cells with IL-6 intervention before lobaplatin treatment