Fig. 6

Expression levels of p-FLNC and its predicted kinases AKT1, p-ERK1/2, p-JNK and p-P38 in SaOS-2 osteosarcoma cells. a Western blot analysis bands showing higher expression of p-FLNC, AKT1, and p-ERK1/2 in the IL-6 group and lower expression of p-JNK and similar expression of p-P38 between the IL-6 group and the Con and Lob groups. Cells were divided into the Con, Lob, and IL6 groups. b ImageJ was used to calculate the density of the bands. The values of each phosphoprotein were normalized against their total proteins, while the value of AKT1 was normalized against GAPDH. Significant differences are indicated. *p < 0.05