Fig. 2

Knockdown of UCA1 suppresses cell proliferation in vitro and tumor growth in vivo. A The RNA level of UCA1 in HCT-116 UCA1 knockdown (KD) cells as determined by qRT-PCR. B UCA1 KD suppresses colony formation of HCT116 cells. Left, the representative picture of cell colonies; right, the statistical result. C UCA1 KD impairs cell proliferation ability of HCT116 cells as determined by CCK-8 assay. D and E UCA1 KD suppresses the tumor growth in vivo. Tumors were harvested at the end of experiment. D the picture of tumors; E the statistical analysis of tumor weight. Error bars represent SD; n = 3 for A ~ C; *P < 0.05; **P < 0.01; ***P < 0.005