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Fig. 3 | Cancer Cell International

Fig. 3

From: Stabilization of UCA1 by N6-methyladenosine RNA methylation modification promotes colorectal cancer progression

Fig. 3

Knockout of m6A writers downregulates UCA1 expression. A Me-RIP and qRT-PCR assays suggest the m6A modification of UCA1 in HCT-116 and DLD-1 cells. B and C Knockout of METTL3 impairs UCA1 expression in HCT-116 cells. B Representative western blot result showed knockout of METTL3 in HCT-116 cells. C downregulation of UCA1 in HCT-116 METTL3 KO cells was detected by qRT-PCR. D and E Knockout of METTL3 impairs UCA1 expression in DLD-1 cells. D, Representative western blot showing knockout of METTL3 in DLD-1 cells. E downregulation of UCA1 in DLD-1 METTL3 KO cells was detected by qRT-PCR. F and G Knockout of WTAP suppresses UCA1 expression in HCT-116 cells. F Representative western blot showing knockout of METTL3 in HCT-116 cells. G, downregulation of UCA1 in HCT-116 WTAP KO cells was detected by qRT-PCR. H and I Knockout of WTAP suppresses UCA1 expression in DLD-1 cells. H, Representative western blot showing knockout of WTAP in DLD-1 cells. I downregulation of UCA1 in DLD-1 WTAP KO cells was detected by qRT-PCR. Error bars represent SD, n = 3, **P < 0.01, ***P < 0.005

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Cancer Cell International

ISSN: 1475-2867

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