Fig. 5

MiR-1224-5p bound to FOXM1 in CRC cells. A StarBase online database showed that the putative binding sites of miR-1224-5p for FOXM1 3′UTR. B, C Luciferase activity was detected by dual-luciferase reporter assay in HCT-116 and SW480 cells. D, E GEPIA and TCGA datasets were employed to assess FOXM1 expression in CRC tissues and normal colorectal tissues. F The mRNA level of FOXM1 was determined by qRT-PCR in 29 pairs of CRC and paracancerous normal tissues. G The linear relationship between miR-1224-5p and FOXM1 expression was analyzed by Spearman correlation analysis. H, I The protein level of FOXM1 was determined by western blot in 29 pairs of CRC and paracancerous normal tissues and NCM460, HCT-116 and SW480 cells. J The impacts of miR-1224-5p mimic and inhibitor on FOXM1 protein expression were revealed by western blot. *P  < 0.05, ***P  < 0.001 and ****P  < 0.0001