Fig. 1

DBF4 is upregulated in GC. A The volcano map shows the expression variations of genes in GC tissues compared to matched adjacent normal tissues from TCGA. B Gene set enrichment analysis (GSEA) of differentially expressed genes [Enrichment Score (ES) = 0.634, Normalized Enrichment Score (NES) = 2.066, Nominal p-value = 0.0, FDR q-value = 0.008] (left) and the heat map shows differentially expressed genes in the KEGG_CELL_CYCLE (right). C Analysis of DBF4 mRNA expression in 375 GC tissues and 32 adjacent tissues. D DBF4 protein expression was detected by western blotting in four paired GC samples. E DBF4 expression in normal cell line GES-1 and GC cell lines MGC-803, AGS, MKN45 and MKN28 was measured by qRT-PCR. Data are displayed as the mean ± SD of three independent experiments. ^**P < 0.01, ^***P< 0.001, ^****P< 0.0001