Fig. 5

The miR-30a inhibits the expression of DBF4. A The heat map (left) and volcano map (right) show significantly downregulated and upregulated miRNAs in GC tissues from TCGA. B A Venn diagram shows the overlap of target miRNAs of DBF4, predicted by TargetScan, Tarbase, and the miRNA prolife from TCGA. C The expression levels of miR-30a in GC tissues from TCGA were analyzed. D The correlation between DBF4 and miR-30a expression in GC tissues was assessed by Pearson’s correlation method. E The expression of DBF4 in MGC-803 and AGS cells transfected with miR-30a or NC was detected by qRT-PCR and western blotting. F Schematic illustration of the binding site between miR-30a and wild-type (WT) or mutant (Mut) DBF4 3ʹ-UTR. G Relative luciferase activity of WT or Mut DBF4 3ʹ-UTR in MGC-803 and AGS cell lines after overexpression of miR-30a. Data are presented as the mean ± SD (n = 3). ^**P < 0.01, ^****P< 0.0001