Fig. 6
From: CircRFX3 contributes to glioma progression through the circRFX3-miR-1179/miR-1229-VASP axis
Overexpression of VASP abrogated the effects of circRFX3 silencing on glioma cell biological behaviors. A The protein level of VASP in vector or VASP transfected A172 and U251 cells was examined by western blot assay. B–F After the transfection of sh-NC, sh-circRFX3#1, sh-circRFX3#1 + vector or sh-circRFX3#1 + VASP, the B viability, C colony formation, D and E migration and invasion, and F apoptosis of A172 and U251 cells were evaluated by CCK-8 assay, colony formation assay, transwell assay and flow cytometry analysis, respectively. *P < 0.05, **P < 0.01