Fig. 2

Stably downregulated NAP1L1 attenuated breast cancer cell proliferation. A RT-qPCR showed that NAP1L1 mRNA expression was upregulated after breast cancer cells were infected with shRNA-NAP1L1 lentivirus compared to negative control (NC) lentivirus. B Western blot analysis showed that the protein level of NAP1L1 was upregulated after the breast cancer cells were infected with shRNA-NAP1L1 lentivirus compared to NC lentivirus. C MTT assay indicated that shRNA-NAP1L1 inhibited the cell proliferation in breast cancer cells. D Knockdown of NAP1L1 expression suppressed the plate clone formation in vitro. E Downregulation of NAP1L1 suppresses EdU staining of breast cancer (scale bar: 200 μm). The data are presented as mean ± s.d. from three independent experiments. *P < 0.05 vs. control; **P < 0.01; ***P < 0.001. F Xenograft tumor of nude mice showed that the average weight and volume of tumors decreased in shRNA-NAP1L1 group compared with the negative control group (n = 5 per group). G Expression levels of PCNA and Ki-67 were measured via IHC staining in the xenograft tumor of nude mice (100 × visual field scale bar: 200 μm, 400e bar: bar: 50 μm). The data are presented as mean ± s.d. from three independent experiments. *P < 0.05 vs. control; **P < 0.01; ***P < 0.001