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Fig. 3 | Cancer Cell International

Fig. 3

From: Starvation induced autophagy promotes the progression of bladder cancer by LDHA mediated metabolic reprogramming

Fig. 3

Starvation-Induced Autophagy Regulated BLCA Glucose Metabolic Reprogramming by Transcriptional Overexpression of LDHA. a Glucose consumption and lactate production ratios were measured in T24 and UM-UC-3 cells after normal or HBSS treatment, homogenized by cell counting as 104 cells (n = 3, unpaired Student’s t test, P ns > 0.05, P* < 0.05, P** < 0.01, P*** < 0.001). b and c Glucose consumption and lactate production ratio in T24 were measured in different groups, including normal, normal supplemented with CQ, starvation only, starvation supplemented with CQ or starvation supplemented with 2-DG. d Western blotting was used to detect the changes in autophagy levels, reflected by P62 and LC3B-II/I expression in T24 cells under normal, normal supplemented with 2-DG, starvation only and starvation supplemented with 2-DG treatment. e Immunofluorescence staining of LC3B was performed in different groups of T24 cells, and the aggregation of LC3B represents the formation of autophagosomes. f Real-time qPCR was performed to determine the relative expression levels of key glycolysis enzymes in T24 and UM-UC-3 cells incubated with HBSS or normal control. g Real-time qPCR and western blot analysis of the transcriptional and translational expression of LDHA in T24 and UM-UC-3 cells with different treatments. β-Actin was chosen as the loading control

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