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Fig. 6 | Cancer Cell International

Fig. 6

From: Starvation induced autophagy promotes the progression of bladder cancer by LDHA mediated metabolic reprogramming

Fig. 6

Starvation Regulated the Expression of LDHA through the Canonical Wnt/β-Catenin Pathway. a GSEA plots showed that the Wnt/β-catenin pathway was involved in LDHA expression in BLCA. b Transcription factor binding sites of LDHA and its location on the chromosome were predicted by USUC and JASPAR. DNA-binding sequences of the transcription factors LEF1 and TCF4 were present in the human LDHA promoter region and were demonstrated with their binding probabilities prediction. c Real-time qPCR was used to determine the expression of LDHA and CTNNB1 at the transcriptional level in T24 cells with normal incubation with DMSO, starvation with DMSO and starvation with PNU74654 (n = 3, unpaired T test, Pns > 0.05, P* < 0.05, P** < 0.01). d Western blots showing TCF4 up- or downstream proteins in T24 cells with different treatments. E Expression of β-catenin in the nucleus and cytoplasm of T24 and UM-UC-3 cells treated with or without starvation. Histone H3 was chosen as the nuclear loading control, while GAPDH was used as the cytoplasmic reference. F Immunofluorescence staining of β-catenin was performed in T24 cells treated with normal, starvation only or starvation supplemented with CQ, and fluorescence localization was detected by confocal microscopy (scale bar, 8 µm). g Western blot analysis presented changes in the expression levels of different proteins in the canonical Wnt/β-catenin pathway in T24 cells under different treatments

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