Skip to main content
Fig. 7 | Cancer Cell International

Fig. 7

From: Starvation induced autophagy promotes the progression of bladder cancer by LDHA mediated metabolic reprogramming

Fig. 7

Starvation Regulated the Wnt/β-Catenin Pathway by Ubiquitination of Axin1 and Promotion of Autophagy. a Changes in the expression levels of different proteins, including the autophagic flux early-stage inhibitor 3-MA and the final-stage inhibitor CQ, in T24 cells after starvation and treatment with autophagy inhibitors at different stages were detected by Western blot. b The amount of ubiquitinated proteins in T24 cells treated with or without HBSS and the expression of Axin1 and P62 determined by western blot. β-Actin was employed as a loading control. c Anti-ubiquitin and anti-Axin1 antibodies were chosen to determine the ubiquitination level of Axin1. d Western blotting was performed in T24 cells incubated with or without HBSS and treated with DMSO or MG132. e Forward and reverse immunoprecipitation assays were chosen to detect the interaction of Axin1 and P62 in T24 cells treated with normal conditions or starvation conditions. F A schematic model of starvation-induced enhanced autophagy and glycose metabolic reprogramming realized by LDHA overexpression in BLCA

Back to article page