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Fig. 3 | Cancer Cell International

Fig. 3

From: Promotion of gastric tumor initiating cells in a 3D collagen gel culture model via YBX1/SPP1/NF-κB signaling

Fig. 3

ITGB1 regulated TICs characteristics through YBX1/SPP1 signaling. A Western blotting of YBX1 and SPP1 in SGC-7901/BGC-823 (Dish), 3D cultured SGC-7901/BGC-823 and 3D cultured ITGB1 KO SGC-7901/BGC-823 cells (KO1, KO2). B Western blotting of YBX1 in SGC-7901/BGC-823 (SCR) and YBX1 KO SGC-7901/BGC-823 cells (KO1, KO2). C Western blotting of SPP1 in SGC-7901/BGC-823 (SCR) and SPP1 KO SGC-7901/BGC-823 cells (KO1, KO2). D The colony growth curves of SGC-7901/BGC-823 and YBX1 KO SGC-7901/BGC-823 cells in 3D collagen gels. The scale bar is 50 μm. E The colony growth curves of SGC-7901/BGC-823 and SPP1 KO SGC-7901/BGC-823 cells in 3D collagen gels. The scale bar is 50 μm. F The colony formation capability of SGC-7901/BGC-823 and YBX1 KO SGC-7901/BGC-823 cells in dish. G The colony formation capability of SGC-7901/BGC-823 and SPP1 KO SGC-7901/BGC-823 cells in dish. H SGC-7901/BGC-823, YBX1 KO SGC-7901/BGC-823 and SPP1 KO SGC-7901/BGC-823 cells were collected and treated with 5-FU (1 µg/ml) for 48 h. The cell apoptosis was examined using Annexin/PI staining. I SGC-7901/BGC-823, YBX1 KO SGC-7901/BGC-823 and SPP1 KO SGC-7901/BGC-823 cells were collected and treated with PTX (2 µg/ml) for 48 h. The cell apoptosis was examined using Annexin/PI staining. J overall survival of gastric cancer patients with high/lowYBX1 expression (low expression, n = 173 and high expression, n = 172). K overall survival of gastric cancer patients with high/low SPP1 expression (low expression, n = 169 and high expression, n = 172)

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Cancer Cell International

ISSN: 1475-2867

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