Fig. 7

ARHGAP25 inhibited MG63 and U2OS cells’ growth, migration and invasion. a ARHGAP25 expression was determined in vector transfected MG63 and U2OS cells. b Overexpression of ARHGAP25 in MG63 and U2OS cells inhibited cell growth. c Representative images of MG63 and U2OS cells wound healing assay at 0 and 24 h after scratching. Bars indicate: 100 µm. d, e Quantification of the gap size in wound healing assay (n = 3), which showed that overexpression of ARHGAP25 suppressed wound healing. f Representative images of MG63 and U2OS cells stained with crystal violet in the transwell assay. Bars indicate: 100 µm. g Counts of cells invaded through the chambers (n = 3). h Representative images of colony formation assay to analyze cell proliferation in ARHGAP25 overexpressed OS cells. i Quantification of the colony number (n = 3). j Protein expression of Twist1, Vimentin, and E-cadherin was measured in OS cells with ARHGAP25 overexpression. k Quantification of protein expression using ImageJ. *Indicates P < 0.05, **indicates P < 0.01 and ***indicates P < 0.001. Picture c, f are magnified 100 times and h is not magnified