Fig. 3

Validation of proteins expression. A The differentially-expressed level of PIWIL4 and SUPT5H were shown in boxplot. B The PIWIL4 and SUPT5H expression level was detected by qRT-PCR, h18S was used as internal control. C Representative IHC images of the PIWIL4 and SUPT5H expression in ICC and para-carcinoma tissues (200 × magnification). D Immunoreactive score (IRS) of the PIWIL4 and SUPT5H in ICC samples and normal tissues. (* P < 0.05; ** P < 0.01; *** P < 0.001, **** P < 0.0001)