Fig. 2

HK2 overexpression promotes distant metastasis of SiHa and HeLa cells in vivo. Female BALB/c nude mice were injected via the tail vein with HeLa-Vec and HeLa-HK2 cells. Representative lung and hematoxylin and eosin-stained images are presented to show tumor lesions in the HeLa-Vec (A) and HeLa-HK2 (B) groups. C The scatter plots show the number of lesions in the lungs as the mean ± SD (n = 6). Female BALB/c nude mice were injected via the tail vein with SiHa-Vec and SiHa-HK2 cells. Representative lung and hematoxylin and eosin-stained images are presented to show tumor lesions in the SiHa-Vec (D) and SiHa-HK2 (E) groups. F The scatter plots show the number of lesions in the lungs as the mean ± SD (n = 6). Female BALB/c nude mice were injected via the tail vein with HeLa-shCtr and HeLa-shHK2 cells. Representative lung and hematoxylin and eosin-stained images are presented to show tumor lesions in the HeLa-shCtr (G) and HeLa-shHK2 (H) groups. I The scatter plots show the number of lesions in the lungs as the mean ± SD (n = 6). Scale bars, 500 μm and 100 μm. Data were statistically analyzed with Student’s t-test, and values are shown as the mean ± SD. **p < 0.01