Table 2 Primers used for gene detection
Gene | Sequence (5′-3′) |
|---|---|
ATF5 | F: TGGCTCGTAGACTATGGGAAA |
R: ATCAACTCGCTCAGTCATCCA | |
GAPDH | F: TGTGGGCATCAATGGATTTGG |
R: ACACCATGTATTCCGGGTCAAT | |
DVL1 | F: GAGGGTGCTCACTCGGATG |
R: GTGCCTGTCTCGTTGTCCA | |
NANOG | F: TCCCGAGAAAAGATTAGTCAGCA |
R: AGTGGGGCACCTGTTTAACTT | |
SOX2 | F: CTCGTGCAGTTCTACTCGTCG |
R: AGCTCTCGGTCAGGTCCTTT | |
ABCG2 | F: CAGGTGGAGGCAAATCTTCGT |
R: ACCCTGTTAATCCGTTCGTTTT | |
TCF1 | F: CAGAGGAGAGGAACCAAGCTA |
R: GCAACTCGGGACATAAAGCC | |
CCND1 | F: GCTGCGAAGTGGAAACCATC |
R: CCTCCTTCTGCACACATTTGAA | |
CD44 | F: CTGCCGCTTTGCAGGTGTA |
R: CATTGTGGGCAAGGTGCTATT | |
JUN | F: CATTGTGGGCAAGGTGCTATT |
R: ACAGAGCGAGTGAAAATGTGTAT |