Fig. 2From: CCR9 initiates epithelial–mesenchymal transition by activating Wnt/β-catenin pathways to promote osteosarcoma metastasisa PPI network of DEGs and a core module of the PPI network. CCR9 was the highest-ranked PPI hub gene. b Immunohistochemistry staining for CCR9 in OS tissues with or without lung metastasis. c CCR9 immunohistochemistry intensity score in OS tissues (21 without lung metastasis and 10 with lung metastasis), and the score was higher in OS tissues with lung metastasis. d Relative CCR9 mRNA expression levels in HD-MSCs, U2OS, HOS, MG63 and SAOS2 cell lines. The expression of CCR9 was higher in OS cell lines. e Protein expression levels of CCR9 in HD-MSCs, U2OS, HOS, MG63 and SAOS2 cell lines. f Quantitative western blot data for CCR9 protein expression. The expression of CCR9 was higher in OS cell lines. Mean ± SD from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001Back to article page