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Fig. 3 | Cancer Cell International

Fig. 3

From: CCR9 initiates epithelial–mesenchymal transition by activating Wnt/β-catenin pathways to promote osteosarcoma metastasis

Fig. 3

a MG63 and HOS cells were transfected with blank control, negative control siRNA (NC) or CCR9 siRNA (si-1, si-2 and si-3). The relative CCR9 mRNA expression levels were obviously lower in si-CCR9 group. b Protein expression levels of CCR9 in MG63 and HOS cells treated with either blank control, si-NC or si-CCR9, as determined by western blot analysis. The protein expression levels were apparently lower in si-CCR9 group. c CCK8 assay results showed no significant difference in two groups. d Colony formation capacity of MG63 and HOS cells, as assessed by a colony formation assay after 10 days. e Quantification of the cell colony number, and the results showed no significant difference in two groups. f The apoptosis rates of MG63 and HOS cells were analyzed by Annexin V-FITC/PI staining. The percentage of apoptotic cells was calculated as the sum of the early apoptotic (Q3) and late apoptotic cells (Q2) percentages. g Quantitative apoptosis rates of MG63 and HOS cells, and no significant difference in two groups. Mean ± SD from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001

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