Fig. 2

EMI2 promotes the proliferation of cholangiocarcinoma cells. A, B The levels of EMI2 in healthy intrahepatic bile duct epithelial HIBepic and cholangiocarcinoma cells (QBC939, RBE, HUCCT1, and HUCC-9810) were detected using qRT-PCR and western blotting; C qRT-PCR was used to detect EMI2 silencing efficiency; D Western blotting was used to detect EMI2 silencing efficiency; E CCK-8 assay was used to detect OD450 in each group; F Cell cloning test was used to detect the proliferation effect; G, H qRT-PCR and western blotting were used to detect the overexpression of EMI2. I, J CCK-8 and colony cloning assays were performed to detect the proliferation of bile duct cancer cells with overexpressed EMI2 (*P < 0.05)