Fig. 2

DMAMCL inhibited tumor growth and prolonged survival in mice bearing NB tumors. BALB/C nude mice were inoculated with NGP and BE2 cells and then treated with DMAMCL (75 or 100 mg/kg) for 21 days. A Tumor volume was measured three times a week and compared at the end of the experiment. Error bar: SE, ns (not significant): control vs DMAMCL treatment, *p < 0.05; **p < 0.01; ***p < 0.001; NGP (n = 10), BE2 (n = 10). B Survival curves of mice bearing NGP and BE2 tumors plotted using Kaplan–Meier analysis. C Representative TUNEL staining images (green) of tumor tissue from mice bearing NGP and BE2 tumors after DMAMCL (75 or 100 mg/kg) treatment for a week. Nuclei were stained with DAPI (blue). The magnification of the images was 200×. Scale bar: 100 μm. D Cleaved PARP expression in NGP and BE2 tumor tissues detected by western blotting, *p < 0.05; **p < 0.01; ***p < 0.001