Fig. 1

HnRNPK is frequently overexpressed in prostate cancer. A Twenty-two formalin-fixed and paraffin-embedded primary prostate cancer tissues were subjected to IHC analyses of the HnRNPK protein. Representative images are shown in normal, low-risk, and intermediate/high-risk prostate cancer tissues. Magnification: × 400 (top) and × 1000 (bottom). B Immunoblot analysis of the HnRNPK protein levels in 27 randomly selected PrCa tissues and paired noncancerous prostate tissues; vinculin was used as an internal control. C HnRNPK mRNA levels in 53 PrCa tissues and paired noncancerous prostate tissues. GAPDH served as loading controls. D Relative HnRNPK mRNA expression levels in PrCa tissues and adjacent normal prostate tissues in a public dataset (GSE70770). E The effect of the HnRNPK expression level on biochemical recurrence in 203 prostate cancer patients who did not undergo androgen deprivation therapy, chemotherapy, radiotherapy or other anticancer treatments was analyzed, and Kaplan–Meier plots were generated using Kaplan–Meier Plotter. F The level of HnRNPK in human prostate cancer cells was detected by RT–qPCR and immunoblot (G). *P < 0.05, **P < 0.01, and ***P < 0.001