Fig. 3

Mature miR-206 and miR-613 expression are decreased in PrCa and directly target the 3’-UTR of HnRNPK to decrease its expression. A Relative miR-206 and miR-613 (C) levels in 53 PrCa tissues and paired noncancerous prostate tissues. U6 served as a loading control. B Scatter diagram showing relative miR-206 and miR-613 (D) expression in PrCa tissues and adjacent normal prostate tissues from a public dataset (GSE21036 and GSE60117). E RT–qPCR analysis of relative miR-206 and miR-613 (F) expression in human PrCa cell lines. G, H Schematic diagram of the predicted target binding sites of miR-206 and miR-613 in the 3’-UTR of HnRNPK. The seed recognition site is denoted. The nucleotides of the 3’-UTR of HnRNPK that binds with miR-206 and miR-613 are highly conserved across species, as predicted by TargetScan (http://www.targetscan.org/vert_71/). I The luciferase activity of the wild-type HnRNPK 3’-UTR (Wt) and mutant HnRNPK 3’-UTR (Mut) cotransfected with miR-206/miR-613 mimics or a miRNA negative control (miR-LacZ) was measured in DU145 cells. Relative luciferase activity was plotted as the mean ± SD of three independent experiments. J, K Expression of HnRNPK in PrCa cell lines transfected with miR-206/miR-613 mimics or inhibitor (L) was detected by RT–qPCR and immunoblot analysis, respectively. Error bars represent the mean ± S. D of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001