Skip to main content
Fig. 6 | Cancer Cell International

Fig. 6

From: Transcriptional regulation and ubiquitination-dependent regulation of HnRNPK oncogenic function in prostate tumorigenesis

Fig. 6

Prostate cancer-associated SPOP mutants impair to interact with and degrade HnRNPK. A A schematic illustration of SPOP domains and prostate cancer-associated mutations. B WB analysis of WCL and immunoprecipitates derived from 293 cells transfected with Flag-HnRNPK and HA-SPOP-WT or deletion of MATH domain-SPOP constructs. 30 h post-transfection, cells were treated with 10 μM MG132 for 10 h before harvesting. EV, empty vector. C WB analysis of WCL derived from 293 cells transfected with indicated plasmids. D WB analysis of WCL and IP derived from 293 cells transfected with HA-SPOP-WT or prostate cancer -associated SPOP mutants. Cells were treated with 10 μM MG132 for 10 h before harvesting. E WB analysis of WCL derived from C4-2 cells stably expressing HA-SPOP-WT or PrCa-associated SPOP mutants. F 293 cells transfected with Flag-HnRNPK together with the indicated HA-SPOP expressing plasmids. 30 h post-transfection, cells were treated with 100 μg/ml CHX for the indicated time period before harvesting. Relative Flag-HnRNPK protein abundance was quantified by Image J and plotted in (G). H WB analysis of WCLs and His pull-down products derived from 293 cells transfected with indicated constructs and treated with MG132 (10 μM) 10 h

Back to article page