Fig. 2

High expression of miR-29c in LLC cells ameliorate muscle catabolism and myotube atrophy. A The expression level of miR-29c is elevated in LLC cells after miR-29c mimic transfection. microRNA level of miR-29c was determined by qRT-PCR analyses. Data (n = 3) were analyzed by Student’s t-test, ***P < 0.001. B, C High expression of miR-29c in LLC cells ameliorate catabolic response in C2C12 myotubes. C2C12 myotubes were treated with a conditioned medium of LLC cells transfected with a miR-29c mimic or its negative control for 48 h. The protein involved in catabolic response were evaluated by western blotting. Data (n = 3) were analyzed by Student’s t-test, *P < 0.05, ***P < 0.001, ****P < 0.0001. D High expression of miR-29c in LLC ameliorate C2C12 myotube atrophy. C2C12 myotubes were treated as described in B, C and analyzed for myotube diameter of MHC-stained myotubes (at 48 h). E High expression of miR-29c in LLC ameliorate C2C12 myotube atrophy. C2C12 myotubes were treated as described in B, C and analyzed for myotube diameter (at 48 h). Data (n = 3) were analyzed by Student’s t-test, ****P < 0.0001