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Fig. 3 | Cancer Cell International

Fig. 3

From: Downregulation of miR-29c promotes muscle wasting by modulating the activity of leukemia inhibitory factor in lung cancer cachexia

Fig. 3

Suppressed expression of miR-29c in LLC cells induce muscle catabolism and myotube atrophy. A The expression level of miR-29c is repressed in LLC cells after miR-29c inhibitor transfection. microRNA level of miR-29c was determined by qRT-PCR analyses. Data (n = 3) were analyzed by Student's t-test, ****P < 0.0001. B, C Suppressed expression of miR-29c in LLC cells induces a catabolic response in C2C12 myotubes. C2C12 myotubes were treated with a conditioned medium of LLC cells transfected with a miR-29c inhibitor or its negative control for 48 h. The protein involved in catabolic response were evaluated by western blotting. Data (n = 3) were analyzed by Student’s t-test, *P < 0.05, **P < 0.01, ****P < 0.0001. D Suppressed expression of miR-29c in LLC induce C2C12 myotube atrophy. C2C12 myotubes were treated as described in B, C and analyzed for myotube diameter of MHC-stained myotubes (at 48 h). E Suppressed expression of miR-29c in LLC induce C2C12 myotube atrophy. C2C12 myotubes were treated as described in B, C and analyzed for myotube diameter (at 48 h). Data (n = 3) were analyzed by Student's t-test, ****P < 0.0001

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Cancer Cell International

ISSN: 1475-2867

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