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Fig. 4 | Cancer Cell International

Fig. 4

From: Downregulation of miR-29c promotes muscle wasting by modulating the activity of leukemia inhibitory factor in lung cancer cachexia

Fig. 4

miR-29c exerts its activity by targeting LIF, a cachexia-inducing factor. A Venn diagram for potential target gene sets of miR-29c from miRWALK (http://mirwalk.umm.uni-heidelberg.de/), TargetScan (http://www.targetscan.org/) miRDB (http://mirdb.org/) and datasets of high expression of cachexia-inducing factors. B Construction of luciferase plasmids containing LIF 3′-UTR or LIF 3′-UTR mutant. C LIF is a potential target gene of miR-29c. Determination of luciferase activity in 293T cells. Data (n = 3) were analyzed by Student’s t-test, ****P < 0.0001, ns P > 0.05. D C2C12 myotubes were treated with a conditioned medium of LLC cells transfected with a miR-29c mimic or its negative control for 48 h. The protein of LIF was evaluated by western blotting. Data (n = 3) were analyzed by Student’s t-test, ***P < 0.001. E C2C12 myotubes were treated with a conditioned medium of LLC cells transfected with a miR-29c inhibitor or its negative control for 48 h. The protein of LIF was evaluated by western blotting. Data (n = 3) were analyzed by Student’s t-test, ***P < 0.001

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