Fig. 2
Morphine promotes cell cycle progression through the G1/S transition and inhibits apoptosis in H460 cells. a Flow cytometry was used to detect the effect of morphine on the cell cycle of H460 cells. b Quantitative analysis of the population of cells in each phase shown in (a) c AV/PI flow cytometry was used to detect the effect of morphine on the apoptosis of H460 cells. d Quantitative analysis of the apoptotic cells shown in (c). e Left panel: Levels of Bcl-2, Bax, cleaved Caspase-3, cleaved Caspase-9, and cleaved PARP were analyzed using Western blotting. Right panel: Quantitative analysis of apoptosis-related protein expression shown in the left panel. H460 cells were treated with 0.1 μg/μL morphine for 48 h, and the control group was treated with NS at the same volume. All data are shown as the means ± SD, *p < 0.05, **p < 0.01