Fig. 4

Effects of inhibitors on the proliferation, migration, and invasion of H460 cells. a Representative images from the Transwell assay using H460 cells treated with morphine and inhibitors. b Quantitative analysis of migrated cells. c Quantitative analysis of the proliferation rate of H460 cells after continuous treatment with morphine and inhibitors. d Representative images of the Transwell assay with H460 cells that were treated with morphine and inhibitors. e Quantitative analysis of invaded cells shown in the preceding panel. In all experiments, cells were treated with the drugs for 48 h. The concentrations of morphine and MNTX were 0.1 μg/μL, the concentration of PP1 was 5 μM, and the control groups were treated with NS and DMSO at the same volumes. All data are presented as the means ± SD, *p < 0.05, **p < 0.01