Fig. 2From: Characterization of a novel glucocorticoid-resistant human B-cell acute lymphoblastic leukemia cell line, with AMPK, mTOR and fatty acid synthesis pathway inhibitionMorphological and biological characteristics of NALM-6/HDR cell line. a Morphology of NALM-6/HDR, NALM-6/HDR-C5, NALM-6/H and their parental NALM-6 cells under phase contrast microscopy (× 400 magnification, scale bar 20 µM). b Wright-Giemsa staining of cells (× 1000 magnification, scale bar 20 µM). c Cell cycle distribution of NALM-6, NALM-6/HDR, NALM-6/HDR-C5, and NALM-6/H cells. Cells were cultured in a 6-well culture plate at 1 × 105/ml, and cell cycle progression was analyzed by PI staining after 48 h. Experiments were performed in triplicate. The percentage of cells in the G0/G1, S and G2/M phase showed no significant difference (P > 0.05) compared with each other. d Growth curves of NALM-6, NALM-6/HDR, NALM-6/HDR-C5, and NALM-6/H cells. Cells were cultured in a 6-well culture plate at 1 × 105/ml in RPMI-1640 medium with 10% FBS and grown for 7 days. Viable cells were counted using trypan blue staining every day. Experiments were performed in triplicate. e G-banding karyotype of NALM-6 and NALM-6/HDR cells. The karyotype according to ISCN (2013) can be described as 46, XY, t(5;12)(q33;p13), t(7;19)(q11.1;p13.3)Back to article page