Fig. 4

Circ_0016760 regulated HIF1A expression via sponging miR-29b in NSCLC cells. A Starbase online database was employed to predict the putative binding sites between miR-29b and circ_0016760. B MicroT-CDS online database was performed to predict the putative binding sequence between miR-29b and HIF1A. C MiR-29b expression was detected by qRT-PCR in A549 and H1299 cells transfected with miR-29b or miR-con. D–G Dual-luciferase reporter assay was conducted to detect luciferase activity in A549 and H1299 cells. H, I RIP assay was carried out to reveal the direct binding relationship between miR-29b and circ_0016760 or HIF1A. J, K The effects between ectopic circ_0016760 expression and miR-29b mimic on miR-29b expression and HIF1A protein expression were demonstrated by qRT-PCR and western blot, respectively, in A549 and H1299 cells. *P < 0.05